PERBAIKAN KUALITAS BAHAN PEMBAWA Rhizobium dan FUNGI PELARUT FOSFAT MELALUI STERILISASI SINAR GAMMA Co-60 DAN PENGARUHNYA TERHADAP PERTUMBUHAN DAN PRODUKSI KEDELAI (Glycine max L.)

Taufiq Bachtiar(1), Iswandi Anas(2), Atang Sutandi(3), Ishak Ishak(4),


(1) Departemen Ilmu Tanah dan Sumber Daya Lahan - Institut Pertanian Bogor
(2) Departemen Ilmu Tanah dan Sumber Daya Lahan - Institut Pertanian Bogor
(3) Departemen Ilmu Tanah dan Sumber Daya Lahan - Institut Pertanian Bogor
(4) Pusat Aplikasi Isotop dan Radiasi - Badan Tenaga Nuklir Nasional
Corresponding Author

Abstract


PERBAIKAN KUALITAS BAHAN PEMBAWA RHIZOBIUM dan FUNGI PELARUT FOSFAT MELALUI STERILISASI SINAR GAMMA Co-60 DAN PENGARUHNYA TERHADAP PERTUMBUHAN DAN PRODUKSI KEDELAI. Varietas unggul kedelai yang dihasilkan dari teknik mutasi radiasi harus didukung oleh teknologi pertanian seperti penggunaan pupuk hayati. Penelitian ini dilakukan untuk mempelajari pengaruh iradiasi gamma Co-60 terhadap 1) sifat kimia bahan pembawa, 2) viabilitas mikrob dalam bahan pembawa, 3) pertumbuhan dan produksi kedelai varietas Mutiara 3. Komposisi bahan pembawa yang diuji meliputi b0= 100% gambut (%w/w), b1= 50% gambut + 50% biochar (% w/w), b2=50% gambut + 50% fosfat alam (% w/w), b3=50% gambut + 25 % biochar + 25 % fosfat alam (% w/w), b4=100% biochar (% w/w). Metode sterilisasi yang digunakan yaitu dengan iradiasi sinar gamma Co-60 (r) dan autoclave (a). Hasil menunjukkan komposisi bahan pembawa dan sterilisasi sinar gamma berpengaruh terhadap sifat kimia bahan pembawa yaitu pH, NO3, dan P tersedia. Viabilitas Rhizobium R35 dan fungi pelarut fosfat FPF4 dengan jumlah populasi tertinggi diperoleh pada bahan pembawa biochar dengan sterilisasi iradiasi sinar gamma.  Penggunaan pupuk hayati dengan bahan pembawa biochar yang disterilisasi dengan iradiasi sinar gamma Co-60 berpengaruh nyata dalam meningkatkan serapan N dan serapan P pada biji, dan hasil tanaman kedelai varietas Mutiara 3.

 



Keywords


sterilisasi, iradiasi, sinar gamma, kedelai, pupuk hayati

References


DAFTAR PUSTAKA

[1] D. Riniasih. Outlook Komoditas Pertanian Tanaman Pangan Kedelai, Pusat Data dan Sistem Informasi Pertanian Kementerian Pertanian, ISSN : 1907 – 1507, Jakarta, 2016.

[2] T. White. Corn Yield and Soybean Production Up in 2017, USDA Reports Winter Wheat Seedings and Grain Stocks also reported https://www.nass.usda.gov/Newsroom/2018/01_12_2018.php

[3] A. Ispandi dan A. Munip, "Efektifitas Pengapuran Terhadap Serapan Hara dan Produksi Beberapa Klon Ubikayu di Lahan Kering Masam", Ilmu Pertanian, Vol. 12, No.2, pp. 125 – 139, 2005.

[4] R. Rosliani, Y. Hilman, dan N. Sumarni. "Pemupukan Fosfat Alam, Pupuk Kandang Domba, dan Inokulasi Cendawan Mikoriza Arbuskula terhadap Pertumbuhan dan Hasil Tana­man Mentimun pada Tanah Masam". J. Hortikultura. Vol.16 No.1 pp. 21-30, 2006.

[5] A. Wijanarko, B.H. Purwanto, D. Shiddieq, D. Indradewa, "Pengaruh Kualitas Bahan Organik dan Kesuburan Tanah Terhadap Mineralisasi Nitrogen dan Serapan N Oleh Tanaman Ubikayu di Ultisol". J. Perkebunan & Lahan Tropika, Vol. 2, No.2, pp. 1-14, 2012.

[6] B.N. Fitriatin, A. Yuniarti, O. Mulyani, F.S. Fauziah, dan M.D. Tiara, "Pengaruh Mikroorganisme Pelarut Fosfat Dan Pupuk P terhadap P Tersedia, Aktivitas Fosfatase, Populasi Mikroorganisme Pelarut Fosfat, Konsentrasi P Tanaman dan Hasil Padi Gogo (Oryza sativa. L.) pada Ultisols", Jurnal Agrikultura, Vol. 20, No. 3, pp. 210-215, 2009.

[7] R.D. Ningsih dan I. Anas, "Tanggap Tanaman Kedelai terhadap Inokulasi Rhizobium dan Asam Indol Asetat (IAA) pada Ultisol Darmaga", Bul. Agron. Vol. 32, No.2, pp. 25 – 32, 2004.

[8] K.G. Cassman, A.S. Whitney, R.L. Fox, "Phosphorus Requirements of Soybean and Cowpea as Affected by Mode of N Nutrition", Agronomy Journal, Vol. 73, pp. 17-22, 1981.

[9] N.K. Arora, E. Khare, and D.K. Maheshwari, "Plant Growth Promoting Rhizobacteria: Constraints in Bioformulation, Commercialization, and Future Strategies", Plant Growth and Health Promoting Bacteria, Vol. 18, pp. 97-116, 2010.

[10] Haryanto, K. Idris, R.I. Kawalusan, dan E.L. Sisworo, "Pengaruh Pupuk Fosfat Alam Pada Tanah Masam Terhadap Pertumbuhan Jagung Serta Serapan N-ZA dan N-Urea", Jurnal Ilmiah Aplikasi Isotop dan Radiasi, Vol. 4, No.2, pp. 130-142, 2008.

[11] M. Jayadi, Baharuddin, B. Ibrahim, "In vitro selection of rock phosphate solubility by microorganism from Ultisols in South Sulawesi, Indonesia", American Journal of Agriculture and Forestry, Vol. 1, No.4, pp. 68-73, 2013.

[12] L. Herrmann and D. Lesueur 2013. "Challenges of formulation and quality of biofertilizers for successful inoculation", Appl Microbiol Biotechnol. Vol. 97, pp. 8859–8873, 2013.

[13] Y. Bashan Y, L.E. de-Bashan, S.R. Prabhu SR, J.P. Hernandez JP, "Advances in plant growth-promoting bacterial inoculant technology: formulations and practical perspectives (1998–2013)", Plant Soil. Vol 378, pp.1–33, 2014.

[14] F. Hazra dan E. Widyati, "Isolasi, Seleksi Bahan Pembawa dan Formulasi Inokulum Thiobacillus spp.", Jurnal Tanah dan Lingkungan, Vol. 9, No.2, pp. 71-76. 2007.

[15] A. Gandhi dan K. Sivakumar, "Impact of vermicompost carrier based bioinoculants on the growth, yield and quality of rice (Oriza sativa L.) C.V. NLR 145", The Ecoscan Vol. 4, No.1, pp. 83-88. 2010.

[16] M. Albareda, D.N. Rodrı´guez-Navarr, M. Camacho, F.J. Temprano. "Alternatives to peat as a carrier for rhizobia inoculants: solid and liquid formulations", Soil Biology & Biochemistry, Vol. 40, pp. 1–9, 2008.

[17] A. Daza, C. Santamaria, D.N. Rodrı´guez-Navarro, M. Camacho, R. Orive, F.J. Temprano. "Perlite as a carrier for bacterial inoculants", Soil Biology & Biochemistry, Vol. 32, pp. 567-572, 2000.

[18] Saranya, P.S. Krishnan, K. Kumutha and J. French, "Potential for Biochar as an Alternate Carrier to Lignite for the Preparation of Biofertilizers in India", Int. Jr. of Agril., Env. and Biotech. Vol. 4, No.2, pp. 167-172, 2011.

[19] C.N. Kelly, F.C. Calderon, V. Acosta-Martinez, M.M. Mikha, J. Benjamin, D.W. Rutherford and C.E. Rostad CE, "Switchgrass Biochar Effects on Plant Biomass and Microbial Dynamics in Two Soils from Different Regions". Pedosphere, Vol. 25, No.3, pp. 329–342, 2015.

[20] P. Tittabutr, K. Teamthisong, B. Buranabanyat, N. Teaumroong, N. Boonkerd, "Gamma Irradiation and Autoclave Sterilization Peat and Compost as the Carrier for Rhizobial Inoculant Production", Journal of Agricultural Science. Vol.12, pp. 59-67. 2012.

[21] Hanudin, B. Marwoto, Hersanti, A. Muharam, "Kompatibilitas Bacillus subtilis, Pseudomonas Flourescens, dan Trichoderma harzianum untuk mengendalikan Ralstonia solanacearum", J. Hor. Vol. 22, No.2, pp. 173-180, 2012.

[22] J.T. Hamilton, F.I. Attia, "Effect of mixtures of Bacillus thuringiensis and pesticide on Plutella xylostella and the parasite Thyraella collaris", J. Econ. Entomol. Vol. 70, No.1, pp.146-148, 1977.

[23] S. Kumar, R.E. Masto, L.C. Ram, P. Sarkar, J. George, V.A. Selvi, "Biochar preparation from Parthenium hysterophorus and its potential use in soil Application", Ecological Engineering., Vol. 55, pp. 67– 72. 2013.

[24] Eviati dan Sulaeman, Analisis Kimia Tanah, Tanaman, Air, dan Pupuk. Balai Penelitian Tanah: Balai Besar Litbang Sumber Daya Lahan Pertanian, Badan Penelitian dan Pengembangan Pertanian, Departemen Pertanian, Bogor. 2009

[25] J.M. Vincent. A Manual for the Practical Study of the Root-Nodule Bacteria. 1.B.P. Handbook No. I5., Blackwett, Oxford, 1970.

[26] E. Hartley, L.G. Gemella, and D.F. Herridge, "Lime pelleting inoculated serradella (Ornithopus spp.) increases nodulation and yield", Soil Biology & Biochemistry, Vol. 36, pp.1289–1294, 2004.

[27] Y. Wei, Y. Zhao, M. Shi, Z. Cao, Q. Lua, T. Yang, Y. Fana, Z. Wei. "Effect of organic acids production and bacterial community on the possible mechanism of phosphorus solubilization during composting with enriched phosphate-solubilizing bacteria inoculation", Bioresource Technology, Vol. 247, pp. 190–199, 2018.

[28] A.E. Berns, H. Philipp, H.D. Narres, P. Burauel, H. Vereecken, W. Tappe. "Effect of gamma-sterilization and autoclaving on soil organic matter structure as studied by solid state NMR, UV and fluorescence spectroscopy", European Journal of Soil Science. Vol. 59, pp. 540–550, 2008.

[29] T. Bisseling, R.C. Van den bos, A. Van kammen, "The effect of ammonium nitrate on the synthesis of nitrogenase and the concentration of leghemoglobin in pea root nodules induced by Rhizobium leguminosarum", Biochimica et Biophysica Acta, Vol. 539, pp.1-11, 1978.

[30] C.H. Cheng, J. Lehmann, M.H. Engelhard, "Natural oxidation of black carbon in soils: changes in molecular form and surface charge along a climosequence", Geochimica et Cosmochimica Acta, Vol. 72, pp. 1598–1610. 2008.

[31] Y. Yao, B. Gao, M. Zhang, M. Inyang, A.R. Zimmerman, "Effect of biochar amendment on sorption and leaching of nitrate, ammonium, and phosphate in a sandy soil", Chemosphere, Vol. 89, pp. 1467–1471, 2012.

[32] Subba-Rao NS, Biofertilizer in Agriculture. New Delhi (IN): Oxford and IBH Publishing Co, New Delhi, 1982.

[33] N. Fahmi, I. Anas, Y. Setiadi, Ishak, "Pengaruh Metode Sterilisasi Radiasi Sinar Gamma Co-60 dan Autoklaf terhadap Bahan Pembawa, Viabilitas Spora Gigaspora margarita dan Ketersediaan Fe, Mn, dan Zn", Jurnal Tanah dan Iklim, Vol. 41, No. 1, pp. 1-8. 2017.

[34] L. Hale, M. Luth, D. Crowley, "Biochar characteristics relate to its utility as an alternative soil inoculum carrier to peat and vermiculite", Soil Biology & Biochemistry, Vol. 81, pp.228-235, 2015.

[35] M.T. Prendergast-Miller, M. Duvall, S.P. Sohi, "Localisation of nitrate in the rhizosphere of biochar-amended soils", Soil Biology & Biochemistr, Vol. 43, pp. 2243-2246, 2011.

[36] L. Hale, M. Luth, R. Kenney, D. Crowley, "Evaluation of pinewood biochar as a carrier of bacterial strain Enterobacter cloacae UW5 for soil inoculation",. Applied Soil Ecology, Vol. 84 , pp. 192–199, 2014.


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DOI: 10.17146/gnd.2019.22.1.4405

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